Photoreceptors by A. Borsellino (auth.), A. Borsellino, L. Cervetto (eds.)

By A. Borsellino (auth.), A. Borsellino, L. Cervetto (eds.)

This quantity or1g1nates from a NATO complicated learn Institute on "Photoreceptors" devoted to M.G.F. Fuortes, held in Erice 1-12 July 1981. The lectures given on the path supplied a common overview of the photoreceptors features in either vertebrate and inver­ tebrate eyes. Elaborating at the most modern hypotheses the lec­ tures additionally further new and fascinating information. as a way to protect the newness and freshness of the subject material and therefore make sure the usefulness of the amount, the authors of their written contributions emphasize extra particular findings in their present examine instead of the academic nature of the lectures truly awarded. The members of this quantity desire to commit their papers to the reminiscence of Mike Fuortes, who has been for lots of of them an inspiring collegue and buddy. 5 papers that don't strictly pertain to the themes of the direction also are integrated within the quantity, the authors couldn't attend the assembly, yet desired to give a contribution an editorial to this memorial volume.

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A. and Checcucci, A. (1975). Paraf1age11ar body (PFB) pigments studied by fluorescence microscopy in Euglena gracilis Plant Sci. , 4, 47-51. A. and Lenci, F. (1977). "In vivo" microspectrofluorometry of photoreceptor pigments in Euglena gracilis. Photochem. Photobio1. 26, 315-318. , Ferrara, R. and Lenci, F. (1974). Red-light induced accumulation of Euglena gracilis. Photochem. Photobio1. 19, 223-226. , Ferrara, R. and Lenci, F. (1976). Action spectra for photoaccumu1ation of green and colorless Euglena: Evidence for identification of receptor pigments.

In the course of evaluating the records, the question arose whether the aD or the BD spectrum (at 500 nm) would better represent the rhodopsin content of the cell. 7, whereas from BD and LD a dichroic ratio of 10 was calculated. Since the last figure was extraordinarily high, it was decided that neither aD nor BD be used, but instead, their average. 8. 008/ ~m was calculated. When compared with Bufo rods (Harosi, 1975), this Xenopus rod contained only about 50% as much rhodopsin as it could. In estimating the II-cis retinal content of the cell, the aD at 365 nm was determined from Fig.

00 "·00 ..... o : .. ~ 0 ," " ........ _...... - L O . O . 063~' . . .. /-... "'. 600" o . ;-(;~. - - - -:::::,,- ---.......... ! I, 400 ! I ! 500 , ! I ! I! 600 WAVE LENGTH (nm) Fig . 9. Spectra from a smaller than average Necturus rod outer segment. The retina was externally bleached and incubated (4 hrs) at room temperature in 1% ethanolic saline containing 30 ~s/ml of II-cis retinal. Sample recordings are 8scan averages (the reference is 16) . The BD spectrum was recorded following a 3-min.

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