Analytical Chemistry Progress by Robert B. Green (auth.)

By Robert B. Green (auth.)

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Apart from a one-step electronic excitation two- or more-step excitations can be carried out (Fig. 17). In case of a IR-UV-excitation the first step must be done in picoseconds in order to obtain the selectivety for the second step, because of the very fast vibrational relaxation 78,79). Considering future experiments in living cells the problem of IR absorption by the water in the cells has to be solved. Some experiments using selective laser excitation on biomolecules have been started. In Table 3 selective processes in nucleic acids are summarized.

The authors recorded resonance R a m a n spectra after photolysis o f HbCO. They used a 10 ns N d : Y A G laser pulse at 530 nm for photolysis and monitored the R a m a n spectra o f the transient species (occurring within 10 ns after photolysis) with a Nz-pumped dye laser. 38 Laser Spectroscopy of Biomolecules Photodissociation was induced in carboxymyoglobin and oxymyoglobin with ps pulses from a N d : Y A G laser at 530 nm by Eisert et al. 6a). The photodissociation occurred in less than 8 ps; the kinetic and structural alterations were measured by following absorbance changes.

Biomolecules like chlorophyll, rhodopsin and hemoglobin can be investigated by visible excitation because of their visible absorption bands. But other biomolecules such as nucleic acids and proteins, absorb only in the UV region (see Fig. 2, too). Action ranges of phototherapy and photochemotherapy also lie in the UV range (290--370 nm) (Chapter 6). The possibilities using lasers in photobiology and in photomedicine depend on the development of laser technology, particularly on whether the laser properties required (such as intensity, bandwidth, pulse or continuous wave operation, pulse length and pulse repetition rate) are available in the wavelength ranges needed.

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